Abstract: The QuEChERS method is optimized to extract pesticide residues from frozen-drying soil samples, including 24 organochlorine pesticides (OCPs) and 8 organophosphorus pesticides (OPPs) as well as atrazine. An approach to simultaneous determination of 33 pesticide residues in soil is established by combining with gas chromatography-tandem mass spectrometry (GC-MS/MS). The results show that the concentration of 33 pesticide residues is 5.0-100 μg/L, in good linearity, with method limit of detection (LOD) 0.09×10^-9-0.52×10^-9, limit of quantitation (LOQ) 0.30×10^-9-1.71×10^-9, relative standard deviation (RSD) of precision 3.7%-17.2%, and recovery 51.6%-118.8%. The method is simple and rapid, characterized by high recovery and sensitivity, and strong resistance to matrix interference, which is suitable for trace analysis and detection of OCPs and OPPs in soil.