Abstract: To investigate the relationships between different land use types and ground substrate texture, soil organic carbon, and total carbon in the Lishu black soil region, soil samples were systematically collected and analyzed using one-way analysis of variance (ANOVA) and multiple comparison methods. The results indicate that the ground substrate texture of soils in Lishu County is predominantly loamy, followed by sandy and clayey fractions. The texture of ground substrates at different depths is influenced not only by land use type but also by topography, geomorphology, and human activities. Various ground substrate textures affect soil pH, total carbon, and organic carbon content differently. Specifically, in dryland and irrigated cropland, the correlation coefficients between soil particle size and pH, total carbon, and organic carbon content gradually decrease with depth, whereas in forestland, those correlation coefficients gradually increase with depth.